Napier grass stunt disease prevalence, incidence, severity and genetic variability of the associated phytoplasma in Uganda

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dc.contributor.authorKawube, Geofrey
dc.contributor.authorTalwana, Herbert
dc.contributor.authorNicolaisen, Mogens
dc.contributor.authorAlicai, Titus
dc.contributor.authorOtim, Michael
dc.contributor.authorKabirizi, Jolly
dc.contributor.authorMukwaya, Anthony
dc.contributor.authorNielsen, Steen Lykke
dc.date.accessioned2024-02-01T11:04:11Z
dc.date.available2024-02-01T11:04:11Z
dc.date.issued2015-04-30
dc.description.abstractThe prevalence, incidence and severity of Napier grass stunt disease (NGSD) caused by phytoplasma on Pennisetum purpureum, the main fodder for livestock under intensive and semi-intensive management systems in Uganda were determined following a field survey carried out in 17 districts. A total of 298 Napier grass fields were visited and NGSD status visually assessed and 1192 samples collected for identification and confirmation of the phytoplasma by polymerase chain reaction (PCR) assays using universal primers P1/P6 nested with R16F2n/R16R2n and, tuf primers 890/340 nested with 835 and 400. From these, 221 PCR products were sequenced and sequences aligned. Napier grass stunt disease is widely spread at an epidemic proportion, with the districts at different risk levels. The most affected districts are in central, East and North parts of the country while those in the west are least affected. Sequence alignments and Blast searches showed that the phytoplasma causing NGSD in Uganda belonged to the phytoplasma group 16SrXI, with single nucleotide sequence variants in a few districts. Therefore, there is a need for development of an area wide NGSD management strategy to contain the disease.en_US
dc.description.sponsorshipThe Uganda National Council for Science and Technology (grant MSI-WA2-2-19-08) and National Crops Resources Research Institute.en_US
dc.identifier.citationKawube, G., Talwana, H., Nicolaisen, M., Alicai, T., Otim, M., Kabirizi, J., ... & Nielsen, S. L. (2015). Napier grass stunt disease prevalence, incidence, severity and genetic variability of the associated phytoplasma in Uganda. Crop Protection, 75, 63-69.en_US
dc.identifier.issnhttp://dx.doi.org/10.1016/j.cropro.2015.04.020
dc.identifier.urihttps://hdl.handle.net/20.500.14270/442
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofseriesCrop Protection;75 (2015) 63- 69
dc.subjectEpidemicen_US
dc.subjectPCRen_US
dc.subjectPennisetum purpureumen_US
dc.subjectRisken_US
dc.subjecttufen_US
dc.subjectVarianten_US
dc.titleNapier grass stunt disease prevalence, incidence, severity and genetic variability of the associated phytoplasma in Ugandaen_US
dc.typeArticleen_US

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