Faculty of Science

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    1,1,1-Trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and its derivatives in marketed Clarius werneri caught from Uganda’s major urban wetlands
    (Journal of Toxicology and Environmental Health Sciences, 2014-07-29) Nnamuyomba, Proscovia; Jolocam Mbabazi; Muhammad Ntale
    The presence of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and derivatives in the Clarius werneri of Uganda’s major urban wetland ecosystems was investigated. Solid dispersion extraction method for extraction, florisil column method for clean-up, gas chromatograph equipped with an electron capture detector (GC-ECD) for analysis and gas chromatograph equipped with mass spectrophotometer (GC-MS) for confirmation of results were used in this study. The major DDT contaminants detected in the samples were p,p’-DDD, p,p’-DDE and p,p’-DDT which were found in 25, 22 and 21% of the samples, respectively. o,p’-DDD was detected in 19% and o,p’-DDT in 13% of the samples. For o,p’-DDE there were no measurable values since the levels were below limit of quantitation (LOQ). The concentrations of DDT derivatives ranged between ND-0.478 µg/kg for p,p’-DDE, ND-0.387 µg/kg for o,p’-DDD, ND-0.476 µg/kg for p,p’-DDD, ND-0.345 µg/kg o,p’-DDT and ND-0.556 µg/kg for p,p’- DDT. The concentration of total DDT in C. werneri was in the range of 1.111 to 1.328 µg/kg dry wt. Generally, all the samples had DDT derivative levels below the maximum residue limit recommended by Food and Agriculture Organisation/World Health Organization (FAO/WHO) Codex Alimentarius Commission.